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Additional file 2 of Histone acetyltransferase NAA40 modulates acetyl-CoA levels and lipid synthesis

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posted on 2022-01-21, 05:13 authored by Evelina Charidemou, Maria A. Tsiarli, Andria Theophanous, Vural Yilmaz, Chrysoula Pitsouli, Katerina Strati, Julian L. Griffin, Antonis Kirmizis
Additional file 2: Figure S2. Silencing of Naa40 in AML12 Hepatocytes. (A) RT-qPCR analysis of expression of Naa40 mRNA levels in scramble and Naa40-KD cells after 48 h of siRNA treatment (n = 3/group). (B) Representative immunoblot run in triplicate of whole-cell extracts using antibodies against NAA40 and β-actin as loading control (n = 3/group). Data are presented as mean ± SEM and analysed by unpaired 2-tailed student’s t-test; *P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Individual values can be found in Additional file 9: Table S8.

Funding

H2020 Marie Skłodowska-Curie Actions European Regional Development Fund and the Republic of Cyprus through the Research & Innovation Foundation Wellcome Trust the Medical Research Council the European Regional Development Fund and the Republic of Cyprus through the Research & Innovation Foundation

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