posted on 2021-02-03, 04:31authored byYa Fan, Jia Wang, Wen Jin, Yifei Sun, Yuemei Xu, Yipin Wang, Xiubin Liang, Dongming Su
Additional file 2: Supplementary Figure 2. CircNR3C2 upregulates HRD1 via sponging miR-513a-3p in breast cancer. a Firefly luciferase activity in MDA-MB-231 cells cotransfected with reporter plasmids (GV272 vectors containing the 3’UTR of HRD1, harbouring the second wild-type miRNA binding site or its mutant) and the indicated oligonucleotides, normalized with renilla luciferase activity. b RT-qPCR showing the relative expression of miR-513a-3p in MDA-MB-231 and BT549 cells transfected with Lenti-NC or Lenti-circNR3C2. c RT-qPCR showing the relative level of circNR3C2 pulled down by control and circNR3C2-specific probe in MDA-MB-231 and BT549 cells. Data were represented as means ± S.D. of at least three independent experiments.
Funding
National Natural Science Foundation of China National Natural Science Foundation of China (CN)