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Additional file 2: Figure S1. of Avian infectious bronchitis virus disrupts the melanoma differentiation associated gene 5 (MDA5) signaling pathway by cleavage of the adaptor protein MAVS

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posted on 13.11.2017 by Liping Yu, Xiaorong Zhang, Tianqi Wu, Jin Su, Yuyang Wang, Yuexin Wang, Baoyang Ruan, Xiaosai Niu, Yantao Wu
Transcriptional stability of eight candidate reference genes in different chicken embryo tissues. Three eleven-day-old SPF chicken embryos were inoculated with PBS. Then, the kidney (A and B), liver (C and D), muscle (E and F), intestine (G and H), lung (I and J) and trachea (K and L) tissues were collected from the embryos 72 h post-inoculation, and RNA was extracted. Candidate reference gene mRNA was amplified by real-time PCR. The transcriptional stability of the candidate reference genes was measured using geNorm and NormFinder software. (A), (C), (E), (G), (I) and (K) are the geNorm analysis results. Average expression stability M of all eight reference genes. The most stably expressed genes have lower M values. (B), (D), (F), (H), (J) and (L) are the NormFinder analysis results. The lower stability value indicates a gene that is more stable. (TIFF 730 kb)

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