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Additional file 1 of Dehydrodiisoeugenol inhibits colorectal cancer growth by endoplasmic reticulum stress-induced autophagic pathways

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posted on 2021-04-11, 03:18 authored by Changhong Li, Kui Zhang, Guangzhao Pan, Haoyan Ji, Chongyang Li, Xiaowen Wang, Xin Hu, Ruochen Liu, Longfei Deng, Yi Wang, Liqun Yang, Hongjuan Cui
Additional file 1: Figure S1. DEH inhibits cell growth of colorectal cancer cells, but not through apoptosis. A. The chemical structure of DEH used in this study. B. The plate colony formation assay was used to evaluate the cellular activity after treatment with DMSO or DEH. The numbers of clone were quantified and shown on the right of the panel. C. Cell cycles of HCT 116 and SW620 cells were investigated via flow cytometry after treatment with or without DEH for 24 h and 72 h. The distribution ratio of G1, S, and G2 of panel C was determined. All the data are means ±S.D. and are representative of three independent experiments. P-value less than 0.05 was considered to be statistically significant. ns: there was no significant difference, ***P < 0.001.

Funding

the National Natural Science Foundation of China the National Key Research and Development Program of China Chongqing Natural Science foundation of Chongqing Chongqing University Innovation Team Building Program funded projects the Fundamental Research Funds for the Central Universities the China Postdoctoral Science Foundation

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