ppat.1009280.s003.tif (6.09 MB)
Additional cytosol-induced iron-associated genes.
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posted on 2021-08-30, 17:33 authored by TuShun R. Powers, Amanda L. Haeberle, Alexander V. Predeus, Disa L. Hammarlöf, Jennifer A. Cundiff, Zeus Saldaña-Ahuactzi, Karsten Hokamp, Jay C. D. Hinton, Leigh A. KnodlerEpithelial cells were infected with mCherry-S. Typhimurium harboring gfpmut3 transcriptional reporters. At 8 h p.i., cells were fixed & stained with Hoechst 33342 to detect DNA. Representative confocal microscopy images show induction of mntH, entC, SL3990, STnc3250, fhuE, SL1802, iroN, fepB, fepA and fhuA promoters in cytosolic bacteria. Green = transcriptional reporter, red = S. Typhimurium, blue = DNA. Scale bars = 10 μm.
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showed increased expressionremain largely unknownphysicochemical conditions encounteredpathogenicity island 1cell microscopic analysiscauses diarrheal diseaseparallel colonization strategiesepithelial cell lysisserovar typhimurium (<scv ), followedinduced expression signatures>- containing vacuoleidentified bacterial genesbacterial genes requiredsalmonella enterica bacterial releasesalmonella epithelial cellsvirulence genescytosolic colonizationzoonotic pathogenunusual lifestyletypically associatedtyphimurium withintyphimurium replicationtwo nichesuccessfully colonizestudy revealssoxs sita seq approachpermissive environmentoxidative stressmnth manganese deprivationmagnesium deprivationintracellular nichegastrointestinal tractfepb extracellular bacteriaepithelial cytosolenvironmental biosensorsentc begins withinbased rnaarchetypical vacuole
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