ppat.1010800.s004.tif (417.45 kB)
Additional characterization of B2M KO cells.
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posted on 2022-12-27, 18:42 authored by Alejandro Matía, Maria M. Lorenzo, Yolimar C. Romero-Estremera, Juana M. Sánchez-Puig, Angel Zaballos, Rafael BlascoA) Immunofluorescence images of HeLa and HeLa B2M KO cells stained with anti-ITGB1 antibody. Scale bars: 20 μm. B) Western blot of whole cell lysates of HAP1 and HeLa cells, showing HAP1 cells do not express AXL protein.
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wide genetic screenstherefore cell survivalscreening top hitsprovide complete resistanceconsistently found βsingle gene knocksignificant gene hitsearly gene expressionfind genes necessaryabsolute infection blockagethus allow cellsdownstream related pathwaysdiv >< pvaccinia virus infectionvaccinia virusb2m geneidentify genesgenes belongingsurvive infectionvirus internalizationvirus entryvirus bindingsurviving cellssignificantly diminishedresults pointrelevant factorreduced probabilityproteins knownpowerful toolsnovel findinghost factorshost factor2 microglobulin&# 946
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