Additional PCA plots demonstrate (A) separation of treatment vs control groups and (B) the top 10 metabolites separating the PCA of the scaled metabolite abundances.

(A) PCA with the Dox samples removed demonstrates separation between treated and control samples at both 6 and 24 hours. (B) The top 10 metabolites show separation in both the first and second principal component. For the first principal component, ethylmalonate and erythritol have a strong influence, suggesting a phenotypic switch between fatty acid and glucose utilization over time, although the direction is unclear. For the second principal component, phosphate and uracil separate the 5FU condition.

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