Adaptive T cell responses in the BAL.

A. Representative flow cytometry plots showing changes in CD4 and CD8 expression in PMA/ionomycin stimulated CD3+ cells in BAL. Two animals shown (MA24 euthanized at 21-dpi, MA27 euthanized at 6-dpi). B-D. Effect of PMA/ionomycin on the frequency of CD4+ CD8+ (DP, B), CD4+ (C) and CD8+ (D) T cells in BAL before and 6-, 14-, and 21-days post SARS-CoV-2 infection. Bars represent mean and standard deviation. E-J. Nightingale Rose Plots (NRPs) showing fold changes in cytokine and surface protein expression compared to baseline (MFI). Yellow = 6-dpi, Purple = 14-dpi, Green = 21-dpi. Size of petals represents magnitude of increase in expression. Distance from one white ring to the next is a 1-fold change. A decrease in expression is represented by a petal size less than the distance between two rings. Two animals shown (MA24 euthanized at 21-dpi, MA27 euthanized at 6-dpi). At 6-dpi, MA24 DP T cell TNF-⍺ MFI is 24x baseline and CD4+ T cell TNF-⍺ MFI is 50x baseline. Graph cutoff is set to a 12-fold change. Panels B-D. Baseline: n = 3 (No Stimulation (Stim)) and n = 2 (Stim), Day 6: n = 4 (No Stim) and n = 4 (Stim), Day 14: n = 2 (No Stim) and n = 2 (Stim), Day 21: n = 2 (No Stim) and n = 2 (Stim).