Activation of IRE1/sXBP1 pathway in the intestinal mucosa and in the mesenteric adipose tissue (MAT) of Crohn’s disease patients.

mRNA levels (qRT-PCR) of ERN1 gene was investigated in the intestinal mucosa (A) and in MAT (B) of CD patients (CD group) compared to control group (CTR group).*P < 0.05 and **p < 0.01 are considered statistically significant versus control group. Western blot analysis of the spliced/activated form of XBP1 was evaluated in the intestinal mucosa of CD patients (CD Group) compared to controls (CTR Group) (C). Each band represents one patient. *P < 0.05 and **p < 0.01 are considered statistically significant versus control group. Immunohistochemical analysis of sXBP1 was performed on paraffin-embedded slides from intestinal mucosa of CD and CTR group. The arrows show labeled epithelial cells by immunohistochemistry. Original magnification 100X (D). AU: arbitrary unit. ASU: arbitrary scanning unit.