ppat.1012036.s004.tif (1.21 MB)

Absolute nucleotide levels of metabolomic screen of CVB3 infected Hela R19 cells.

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posted on 2024-03-08, 18:21 authored by Lonneke V. Nouwen, Martijn Breeuwsma, Esther A. Zaal, Chris H. A. van de Lest, Inge Buitendijk, Marleen Zwaagstra, Pascal Balić, Dmitri V. Filippov, Celia R. Berkers, Frank J. M. van Kuppeveld

Metabolomics study of mock and CVB3 infected HeLa R19 cells (three replicates; one experiment; MOI 5). The cells were infected, lysed at 2, 4, 6 or 8 hpi and measured by LC-MS to identify metabolites. The absolute levels of nucleotide mono-, di- and triphosphates are shown. The p-values of the absolute metabolite levels were calculated using linear mixed effect models with an interaction of time and treatment and a random effect of replicate. A rank transformation on the data was performed to ensure a normal distribution of the residuals. Afterwards, a contrast analysis was done to calculate the p-values between specific groups. *p < 0.05, **p < 0.01, ***p < 0.001.

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