Absolute HIV RNA copies from CD26 versus CD36 columns in in vitro infection.

MDM or PBMCs were infected with NL4–3(AD8) macrophage tropic HIV able to infect both cell types. Supernatant from the infected cells was collected and diluted to 10⁴ HIV RNA copies/mL. Half the diluted supernatant was applied to CD26 binding columns and the other half to CD36 binding columns to quantify the number of CD26 and CD36 expressing virions. Shown are median and IQR for different blood donors. Macrophage values: CD26 median 208 HIV RNA copies/mL (85–440 copies/mL), CD36 median 5403 copies/mL (3076–9263 copies/mL). PBMC values: CD26 median 6600 copies/mL (5749–11185 copies/mL), CD36 median 5862 copies/mL (3309–5862 copies/mL). p-values are: *< 0.05; **< 0.01; ****< 0.0001 by Kruskal-Wallis non-parametric test with Dunn multiple comparisons correction.

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