pntd.0011878.s010.tif (457.09 kB)
A middle image of Z-stacks (>12 images) with the FITC channel (probe pB-383), showing P. aeruginosa cells inside Acanthamoeba trophozoites instead of sitting on the surface.
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posted on 2024-01-02, 19:15 authored by Binod Rayamajhee, Mark Willcox, Fiona L. Henriquez, Ajay Kumar Vijay, Constantinos Petsoglou, Gauri Shankar Shrestha, Hari Kumar Peguda, Nicole CarntWhite arrows indicate rod-shaped bacterial cells and red line (dotted) represents trophozoite plasma membrane border.
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primary human macrophagesincreasingly important pathogenwild type strainstrain pao1 (<increased neutrophil responsealkyne – functionalizednaturally acquired intracellulardomestic tap waterpseudomonas aeruginosa live intracellular bacteriarats eyes resulteddomestic watermotile strainaeruginosa – alanineclinical responsehousehold waterintracellular survivalintracellular bacteriavivo situ p acanthamoeba various microorganismsspecies recoveredseen throughoutpotentially attributedmetabolically activemay indicategreater degreegenotype t4ffish ),findings indicateenvironmental hostelectron microscopycorneal swabscorneal infectionschallenged intrastromallyanimal modelalso becomingalkdala ).ak patients>< 005 ).
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