A KSHV mutant with K-RTA defective in OTUD4 recruitment showed impaired lytic replication.

(A) SLK.iBAC-K-RTA-WT or SLK.iBAC-K-RTA-F689A/R690A/D691A (K-RTA-3A) cells were induced with Dox (1 μg/ml) for 48 h, followed by co-immunoprecipitation and immunoblotting. (B) SLK.iBAC-K-RTA-WT or SLK.iBAC-K-RTA-3A cells were induced with Dox (1 μg/ml) for 48 h, and WCLs were collected and analyzed by immunoblotting. (C) SLK.iBAC-K-RTA-WT or SLK.iBAC-K-RTA-3A cells were induced with Dox (1 μg/ml) and sodium butyrate (0.5 mM) for 48 h, and KSHV infectious units were quantified. (D) SLK.iBAC-K-RTA-WT or SLK.iBAC-K-RTA-3A cells stably transduced with OTUD4 WT/C45A were induced with Dox (1 μg/ml) and sodium butyrate (0.5 mM) for 48 h, and KSHV infectious units were quantified. WCLs were collected and analyzed by immunoblotting.