ARG2, RGS4, and RGS5 expression in BHP18-21v cells analyzed using real-time PCR and western blotting.

(A) MOI-dependent changes in gene expressions quantified by real-time PCR 72 h after adenoviral vector infection (n = 6). Ratios of gene expressions in infected cells to those in non-infected cells 72 h after infection are shown. (B) Time-dependent changes in gene expressions quantified by real-time PCR after 300 MOI adenoviral vector infection (n = 6). Ratios of gene expressions in infected cells to those in pre-infected cells (0 h) are shown. Error bars indicate SEM. *p<0.05 and **p<0.01, significant differences between AdNKX2-1 and AdLacZ. (C) Western blot showing ARG2 and RGS4 expression 72 h after 300 MOI AdLacZ or AdNKX2-1 infection (upper panel). Loading control, GAPDH expression (bottom panel).