ACs were successfully generated from S2 cells, and the RNAi knockdown efficiency was confirmed by qPCR.

A. S2 cells and ACs were stained with annexin V-FITC and propidium iodide (PI). Scale bar = 20 μm. ACs were obtained from S2 cells after Actinomycin D induction for 18 h, as described in the Materials and Methods section. B. The apoptosis rates of S2 cells and ACs were analyzed by flow cytometry. C. Western blotting detected the Dcp1 protein levels in S2 cells and ACs. Actin was used as the loading control. D. The relative mRNA levels of 24 genes in RNAi-treated S2 cell lines were quantified by qPCR and compared to those of control-treated S2 cells; RpL32 was used as the internal control. Statistical significance was assessed using the two-way ANOVA.

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