A2 deletion suppressed EAE-induced upregulation of F4/80 cells.

A-D) Representative confocal images of optic nerve sections immunostained using F4/80 antibody. WT EAE optic nerves showed an increase in macrophage/monocyte, while A2 deletion greatly suppressed this effect. E-H) Magnified images of boxed areas represent increase in the presence of F4/80 positive cells induced by EAE. I) Quantification of F4/80 positive cells demonstrate the impact of A2 deletion on the number of macrophages induced by EAE. Optic nerve sections stained using antibodies against CD 16/32 (J-M), and CD 86 (O-R) and quantification (N&Q) showed upregulation of M1 macrophages in WT EAE samples which was significantly reduced by the A2 deletion. Studies using an M2 marker, A1, did not show any significant changes across the groups (T-W) Number of animals used in the study: Number of animals used: 8(WT Control);12 (WT EAE); 9 (A2^-/- EAE); 7 (A2^-/- Control). Representative images are presented. Scale bar 50 μm. Data are presented as Mean ± SEM. #p<0.01 WT EAE vs WT Con and # p<0.01 A2^-/- EAE vs WT EAE.