Jem2051593f04.jpg (25.23 kB)
60 μg protein were separated by precast NuPAGE Novex 4–12% Bis-Tris gels and transferred onto nitrocellulose membranes using the XCell II blot system
figure
posted on 2011-12-31, 19:25 authored by Michael Bacher, Richard Dodel, Bayan Aljabari, Kathy Keyvani, Philippe Marambaud, Rakez Kayed, Charles Glabe, Nicole Goertz, Anne Hoppmann, Norbert Sachser, Jens Klotsche, Susanne Schnell, Lars Lewejohann, Yousef Al-AbedThe activation of glial cells was assessed by staining for the macrophage activation with antibodies against the F4/80 antigen. Immunoblot analysis revealed a decline of F4/80 in all CNI-1493 animals. Equal protein loading was assessed by reprobing the membrane with monoclonal antibodies against GAPDH.
Copyright information:
Taken from "CNI-1493 inhibits Aβ production, plaque formation, and cognitive deterioration in an animal model of Alzheimer's disease"
The Journal of Experimental Medicine 2008;205(7):1593-1599.
Published online 7 Jul 2008
PMCID:PMC2442637.
