3D7_1365800 an AH domain containing protein is dispensable for asexual parasite development.

(A) Live cell microscopy images of parasites endogenously expressing PF3D7_1365800-2xFKBP-GFP-2xFKBP by live-cell microscopy across the intra-erythrocytic development cycle. Nuclei were stained with DAPI. Scale bar, 5 μm. (B) Expression of PF3D7_1365800-2xFKBP-GFP-2xFKBP with episomally expressed mCherry-K13. Scale bar, 5 μm. (C) Live-cell microscopy of knock sideways (+ rapalog) and control (without rapalog) PF3D7_1365800-2xFKBP-GFP-2xFKBP^endo+1xNLSmislocaliser parasites or PF3D7_1365800-2xFKBP-GFP-2xFKBP^endo+LYNmislocaliser parasites 16 hours after the induction of knock-sideways by addition of rapalog. Scale bar, 5 μm. (D) Individual growth curves of PF3D7_1365800-2xFKBP-GFP-2xFKBP^endo+1xNLSmislocaliser or PF3D7_1365800-2xFKBP-GFP-2xFKBP^endo+LYNmislocaliser parasites with (red) or without (blue) addition of rapalog. Relative growth of knock sideways (+ rapalog) compared to control (without rapalog) PF3D7_1365800-2xFKBP-GFP-2xFKBP^endo+1xNLSmislocaliser (blue) or PF3D7_1365800-2xFKBP-GFP-2xFKBP^endo+LYNmislocaliser (red) parasites over five days. Three (NLSmisloc) or six (LYNmisloc) independent growth experiments were performed and mean relative parasitemia ± SD is shown. (E) Live cell microscopy images of parasites expressing PF3D7_1365800-2xFKBP-GFP-2xFKBP with episomally expressed Golgi marker GRASP-mCherry. Schematic representation of the cell lines depicted above the corresponding panel. Scale bar, 5 μm.

(TIF)