posted on 2011-12-31, 10:43authored bySteven M. Claypool, Yavuz Oktay, Pinmanee Boontheung, Joseph A. Loo, Carla M. Koehler
(A) RCRs, (B) state III respiratory rates in the presence of 50 μM ADP, (C) uncoupled respiratory rates in the presence of 10 μM CCCP, (D) percent inhibition by 40 μM atractyloside compared with state III rates (mean ± SEM). (E) ADP:O ratios in the presence of 2 mM NADH, (F) ATP production (percentage of wt) by complex V in the presence of 5 mM NADH, 2 mM ADP, and 10 μM adenylate kinase inhibitor, ApA (mean ± SEM). Aliquots taken at 30, 60, 90, 120, and 150 s after addition of 2 mM ADP were extracted with PCA and ATP levels measured. Unless indicated by “NS”, all differences compared with wt are statistically significant (P ≤ 0.05) by test. = 6–11 (A–D), = 5–9 (E), and = 4 (F).
Copyright information:
Taken from "Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane"