19-BJB induces cell cycle arrest in T24 cells.

(A) FACS analysis data of T24 cell cycle. Samples were analyzed on a BD FACScan flow cytometer and the percentage of cells in the Sub-G1, G0-G1, S, and G2-M phases of the cycle was determined using FACS Express V3. (B) The change of Sub-G1 phase in FACS analysis affected by 19-BJB. (C) T24 cells treated with 19-BJB (0, 2, 4 μM) for 24 h. (D) T24 cells treated with 19-BJB (0, 2, 4 μM) for 48 h. The data are expressed as means ± SD from triplicate samples for each treatment. *p < 0.05, ****p < 0.0001.