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Table 2.xls (5.5 kB)

qPCR master mix for the COVID dust assay.

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posted on 2024-02-09, 18:25 authored by John Van Dusen, Haley LeBlanc, Nicholas Nastasi, Jenny Panescu, Austin Shamblin, Jacob W. Smith, Michael G. Sovic, Amanda Williams, Mikkel B. M. Quam, Seth Faith, Karen C. Dannemiller

The per plate column corresponds to 110 wells per plate to account for reagent loss. The Exo reagents listed are part of the TaqMan Exogenous Internal Positive Control reagent kit (Applied Biosystems, Waltham MA).

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    utilized wastewater samplinginfer community trendsgenome sequencing protocolensure public healthosu &# 8217monitor circulating variantsaverage estimated frequencyprimary variant presentestimated frequencyprimary variantvariants presentvariant compositionviral abundancetrack covidthree variantssurrounding populationscale resolutionresults supportrelative abundancesrecent effortslevel inferencesjanuary 2022earliest sampledominant straincolumbus campuscampus correlatesbased whole19 variants100 %.02 ).(&# 177

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