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pntd.0010777.s002.xlsx (105.56 kB)
S. stercoralis genes (n = 328) differentially expressed in gut-dwelling (dark blue) versus non-gut-dwelling (light blue) life stages of the nematode with those containing predicted epitopes indicated.
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posted on 2023-04-17, 17:36 authored by Tegwen Marlais, Jack Bickford-Smith, Carlos Talavera-López, Hai Le, Fatima Chowdhury, Michael A. MilesTranscriptomic data from Stoltzfus et al. (2012), gut and non-gut groupings and differential analysis from the present study. Gene accession numbers (starting SSTP) are given for WormBase ParaSite or UniProtKB and life stage transcriptomic data accession numbers (starting ERR) for NCBI SRA. Expression levels are in normalized read counts where red is high and white is low.
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multiple sequence alignmentmany parasitic infectionsdiv >< pdescribe 22 candidates>, giving 13d structure modellingassociated protein familiesfive protein familiesused online predictorsspecific diagnostic assaysrevealed 328 proteinsstrongyloides ratti strongyloides stercoralis strongyloides stercoralis protein coproantigensfive parasitismdiagnostic coproantigens>- specificvalidating curetargeted analysisseven genesscreening priorproteomic datasetsproteomic dataprolyl oligopeptidasepowerful approachpotentially fatalpopulation surveillancepartially dueleast homologyintestinal nematodeinfected stoolinadequate diagnosisimmunosuppressed individualshuman infectionepidemiological datadifferentially expressedcompared phylogeneticallyaspartic peptidaseaccelerate development057 orthologues
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