Comprehensive analysis of B3 family genes in pearl millet (Pennisetum glaucum) and the negative regulator role of PgRAV-04 in drought toleranceUntitled Item

Supplementary Table 1 The list of Primer used in this study

Supplementary Table 2 The list of Identification and analysis of PgB3s

Supplementary Table 3 The list of B3 homologous genes that have been annotated

Supplementary Table 4 The list of Arabidopsis thaliana as a homologous species for interactions analysi of PgB3s proteins

Supplementary Table 5 The list of mean FPKM values of PgB3s at different time points under drought treatment

Supplementary Table 6 The list of mean FPKM values of PgB3s at different time points under high-temperature treatment

Supplementary Table 7 The physiological experiment data of three transgenic lines and the WT plants

Figure S1 Phylogenetic relationship of the B3 superfamilies of At (Arabidopsis thaliana), Os (Oryza sativa), and Pg (Pennisetum glaucum). The four colors represent four different subfamilies of the B3 family.

Figure S2 Transformation process of transgenic tobacco. A: preincubate; B: induction; C: screening; D: differentiation; E: rooting.

Figure S3 Identification of transgenic tobacco by different techniques. A: PCR identification of DNA extracted from transgenic plants, M: DL2000 DNA Marker; 1~18 is the PgRAV-04 OE plants; + is a positive control; − is a negative control; WT is a wild-type. B: RT-PCR identification of genetically modified tobacco，OE1-OE6 is PgRAV-04 OE plants, WT is a wild-type. C: Real-time fluorescence quantitative PCR identification of transgenic tobacco, Actin was amplified as an internal reference gene. Data are mean ± SD of three replicates