Stopped-flow analysis of GroEL R231W in the presence of AMP-PNP.

Name: Stopped-flow analysis of GroEL R231W in the presence of AMP-PNP.
Published: 2013-10-30T19:52:53Z

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Stopped-flow analysis of GroEL R231W in the presence of AMP-PNP.

2013-10-30T19:52:53Z (GMT) by Toshifumi Mizuta Kasumi Ando Tatsuya Uemura Yasushi Kawata Tomohiro Mizobata

A. 5-sec assay. The black trace represents fluorescence changes upon addition of ATP, the gray trace represents fluorescence changes upon addition of AMP-PNP. B. 100-sec assay. Legends are as in A.

Keyword(s)

Dynamic Process chaperonin function GroEL apical domain GroEL D 398A variants apical domain movement GroEL rings GroEL SR ATP binding circularly permuted GroEL polypeptide ends GroES binding Escherichia coli GroEL Kinetic analyses CP GroEL mutants Helix M ATP binding site

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