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Stopped-flow analysis of GroEL R231W in the presence of AMP-PNP.

Name: Stopped-flow analysis of GroEL R231W in the presence of AMP-PNP.
Published: 2013-10-30T19:52:53+00:00
Keywords: Dynamic Process, chaperonin function, GroEL apical domain, GroEL D 398A variants, apical domain movement, GroEL rings, GroEL SR, ATP binding, circularly permuted GroEL, polypeptide ends, GroES binding, Escherichia coli GroEL Kinetic analyses, CP, GroEL mutants, Helix M, ATP binding site

dataset

posted on 30.10.2013, 19:52 by Toshifumi Mizuta, Kasumi Ando, Tatsuya Uemura, Yasushi Kawata, Tomohiro Mizobata

A. 5-sec assay. The black trace represents fluorescence changes upon addition of ATP, the gray trace represents fluorescence changes upon addition of AMP-PNP. B. 100-sec assay. Legends are as in A.

Stopped-flow analysis of GroEL R231W in the presence of AMP-PNP.

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