Peak viremia and presence of neutralizing anti-WNV antibodies (nAb) in sera of HOFIs before infection and on day of death.

All HOFI sera were tested for nAb at a 1:20 dilution before infection and at the time of death with the end of the project at 14 dpi. Neutralization of >90% of 50–90 PFU was considered positive using a plaque reduction neutralization assay (PRNT₉₀). Peak viremia titers determined by plaque assay titration are shown as log₁₀ PFU/mL and occurred on 3 dpi or 4 dpi.

Peak viremia and presence of neutralizing anti-WNV antibodies (nAb) in sera of HOFIs before infection and on day of death.