Knockdown of Hsc70 reduces B12-HA DJANGOS.

Hela cells expressing B12-HA were seeded onto coverslips and transfected with siRNAs directed against GFP or Hsc70. Three days later, the coverslips were processed for anti-B12 immunofluorescence. The fraction of cells with DJANGOS was determined in at least 10 random fields for each condition. Three independent experiments were performed using a pool of siRNAs (experiment 1) or two different siRNAs directed against Hsc70 (experiments 2 and 3). Hsc70 siRNA reduced Hsc70 mRNA ∼50 fold.