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Characterization of circulating precursors cells (A-D).

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posted on 2015-12-04, 08:45 authored by Rossana Girardello, Stefano Tasselli, Nicolò Baranzini, Roberto Valvassori, Magda de Eguileor, Annalisa Grimaldi

Morphological (A) and immunohistochemical (B) analysis of cryosections from H. medicinalis body wall. The circulating precursors (arrowhead) visible in lumen of neo-vessels are highly positive for anti-CD45 antibody. Nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI; blue). (C-D) Western blot analysis. A protein extracts from the body wall of untreated (n.t) and MWCNTs treated leeches from 1 to 12 hours and from 1 to 5 weeks were probed with the anti-CD45 antibody. The housekeeping protein D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. In all samples, the anti-CD45 detected specific immunoreactive band 24 of about 145 kDa (C), according to the molecular weight ladder (kDa). CD45 protein was quantified by densitometry from three experiments. *P<0.05 compared with untreated leeches (D). Bars in A-B: 10μm.