Video recording of freely diffusing 60 nm and 80 nm gold nanoparticles using dark field microscopy
Dark field recordings of 60 nm and 80nm gold particles which were diluted in essentially particle-free double distilled H2O. The temperature was 24 °C. The concentration was amounted to approximately 3x10^9 ml.
The video file was captured with a Canon EOS 5D Mark II camera at 25 FPS mounted on a Olympus BX51 microscope (100-fold oil immersion objective) which was illuminated with a CytoViva dark field oil condenser.
The video pixel size is 182 nm.
This video is part of a puplication:
Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x