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TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness

Name: TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
Published: 2024-06-11T06:22:12+00:00
License: https://rightsstatements.org/vocab/UND/1.0/
Keywords: raw sequence reads

dataset

posted on 2024-06-11, 06:22 authored by USDA-ARS, QB3 Berkeley Genomics

We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-04-28

Theme

Non-geospatial

ISO Topic Category

biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

Public Access Level

Public

Accession Number

PRJNA629015

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It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA629015 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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raw sequence reads

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