Three bacterial genomes run on R6 Oxford Nanopore MinIon Chemistry

Three bacterial genomes pooled at equal DNA concentrations and run on R6 Oxford Nanopore Chemistry. Borellia burdorferi B31, Escherichia coli K12 and Streptomyces avermitilis MA 4680.

 

Note that these have been run together as a pool with no barcoding.

 

The Oxford Nanopore reads are contained in the file ninthtranche_renamed.fastq. The remaining FASTA files are the corresponding reference genomes included for convenience. The Lambda Control DNA spike-in sequence is also included. 

 

Bioanalyser traces and materials and methods may be found in the attached file. 

 

Version 1.91 Meterichor basecaller using 2D option.

S.avermitilis 1.8x coverage
B.burgdorferi 18.1x coverage
Lambda control spike-in 1,132x coverage
E.coli 4,64kb 6702 14.2% 0.25x coverage

 

Statistics for read lengths:

Min read length: 5
Max read length: 98,366
Mean read length: 1998.91
Standard deviation of read length: 2942.24
Median read length: 1,040

Statistics for numbers of reads:
Number of reads: 47,093

Non-aligned reads 18051 38%
Aligned reads 29042 62%

Number of reads >=1kb: 24,392

 

Statistics for bases in the reads:

Number of bases in all reads: 94,134,729
Number of bases in reads >=1kb: 82,118,285
GC Content of reads: 47.21 %

 

Organism Genome size Number of reads aligning Percentage of aligned reads Mean depth of coverage