Supporting data for "CRISPR-based applications in next-generation diagnostic and pathogenesis in enteroviruses"

Clustered regularly interspaced short palindromic repeats (CRISPR) is a versatile gene-editing technology that is widely used in life science research to improve human health. The advancement of CRISPR technologies provided comprehensive information on the pathogenesis of emerging infectious pathogens and their potential applications in rapid detection. In this study, two CRISPR-Cas technologies were being employed in an attempt to improve the diagnosis and elucidate the pathogenesis in enteroviruses.

In the first part of my study, CRISPR-Cas9-based genome-wide functional screenings were conducted to discover potential host factors for the pathogenesis of enteroviruses.

In the second part of my study, integration of multiplex RT-LAMP and CRISPR-Cas12a was introduced for the development of a rapid diagnostic tool for EV infection.