ic9b00568_si_001.zip (46.65 MB)
Studies of Redox Cofactor Pyrroloquinoline Quinone and Its Interaction with Lanthanides(III) and Calcium(II)
dataset
posted on 2019-06-11, 15:49 authored by Henning Lumpe, Lena J. DaumannRecently it was discovered
that lanthanides are biologically relevant and found at the centers
of many bacterial proteins. Poorly understood, however, is the evolutionary
advantage that certain lanthanides might have over calcium at the
center of methanol dehydrogenase enzymes bearing redox cofactor PQQ.
Here, we present a straightforward method to obtaining clean PQQ from
vitamin capsules. Furthermore, we provide full NMR, IR, and UV–vis
spectroscopic characterizations of PQQ. We conducted NMR experiments
with the stepwise addition of diamagnetic and paramagnetic lanthanides
to evaluate the binding to PQQ in solution. This study provides a
deeper understanding of PQQ chemistry and its interaction with lanthanides.