Source Data for Figure 6 of Jimenez et al. Methods 2020

Localization files for Figure 6 of:

Jimenez, A., Friedl, K., Leterrier, C. (2020). About samples, giving examples: Optimized Single Molecule Localization Microscopy Methods 174(), 100-114.

Multicolor STORM and DNA-PAINT images of immunostained COS cells. A. 3-color image obtained by sequential acquisition of actin by STORM (phalloidinAF647, gray in overlay), microtubules (two anti-α-tubulin antibodies, green on overlay) and clathrin-coated pits (anti-clathrin light chain, red on overlay) by DNAPAINT (Atto655 imager). Bottom panels are zoomed images corresponding to the highlighted area on the full image. B. 5-color image obtained in three steps: first DNA-PAINT of actin (phalloidin-Atto488, green in overlay), microtubules (two anti-α-tubulin antibodies, Atto565 imager, magenta on overlay) and mitochondria (anti TOM20, Atto655 imager, red on overlay), then DNA-PAINT of intermediate filaments (anti-vimentin, Cy3B imager, blue on overlay), then DNA-PAINT of clathrin (anti-clathrin light chain, Cy3B imager, cyan on overlay).