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CC-BY.txt (12.42 kB)
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Jimenez et al Figure6.pdf (2.72 MB)
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Readme.txt (2.29 kB)
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Locs_Fig6A_ch1_actin_405-647_14895K_TS2D.csv (822.8 MB)
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Locs_Fig6A_ch2_tubulin_647_4383K_TS2D.csv (244.78 MB)
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Locs_Fig6A_ch3_clathrin_647_3957K_TS2D.csv (220.71 MB)
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Locs_Fig6B_ch1_actin_488_1830K_TS2D.csv (100.96 MB)
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Locs_Fig6B_ch2_tom20_560_4950K_TS2D.csv (275.07 MB)
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Locs_Fig6B_ch3_tubulin_647_5652K_TS2D.csv (313.07 MB)
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Locs_Fig6B_ch4_vimentin_560_174K_TS2D.csv (9.44 MB)
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Locs_Fig6B_ch5_clathrin_647_820K_TS2D.csv (44.8 MB)
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Rec_Fig6A_ch1_actin_405-647_14895K_TS2D.tif (25 MB)
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Rec_Fig6A_ch2_tubulin_647_4383K_TS2D.tif (25 MB)
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Rec_Fig6A_ch3_clathrin_647_3957K_TS2D.tif (25 MB)
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Rec_Fig6B_ch1_actin_488_1830K_TS2D.tif (25 MB)
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Rec_Fig6B_ch2_tom20_560_4950K_TS2D.tif (25 MB)
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Rec_Fig6B_ch3_tubulin_647_5652K_TS2D.tif (25 MB)
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Rec_Fig6B_ch4_vimentin_560_174K_TS2D.tif (25 MB)
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Rec_Fig6B_ch5_clathrin_647_820K_TS2D.tif (25 MB)
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Source Data for Figure 6 of Jimenez et al. Methods 2020
Localization files for Figure 6 of:
Jimenez, A., Friedl, K., Leterrier, C. (2020). About samples, giving examples: Optimized Single Molecule Localization Microscopy Methods 174(), 100-114.
Multicolor STORM and DNA-PAINT images of immunostained COS cells. A. 3-color image obtained by sequential acquisition of actin by STORM (phalloidinAF647, gray in overlay), microtubules (two anti-α-tubulin antibodies, green on overlay) and clathrin-coated pits (anti-clathrin light chain, red on overlay) by DNAPAINT (Atto655 imager). Bottom panels are zoomed images corresponding to the highlighted area on the full image. B. 5-color image obtained in three steps: first DNA-PAINT of actin (phalloidin-Atto488, green in overlay), microtubules (two anti-α-tubulin antibodies, Atto565 imager, magenta on overlay) and mitochondria (anti TOM20, Atto655 imager, red on overlay), then DNA-PAINT of intermediate filaments (anti-vimentin, Cy3B imager, blue on overlay), then DNA-PAINT of clathrin (anti-clathrin light chain, Cy3B imager, cyan on overlay).
