Single-cell RNA-seq data from Smart-seq2 sequencing of FACS sorted cells (v2)
datasetposted on 20.09.2018 by Tabula Muris Consortium, James Webber, Joshua Batson, Angela Pisco
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Gene-count tables for FACS sorted cells sequenced with Smart-Seq2 from 20 organs of 7 mice. Cells are grouped by tissue of origin.
Includes data for 53,760 cells, 44,879 of which passed a QC cutoff of at least 500 genes and 50,000 reads.
Cell annotations using the Cell Ontology  controlled vocabulary are in a separate csv.
This differs from v1 by renaming "Brain_Neurons" --> "Brain_Non-microglia" to be consistent with the manuscript.
Update 2018-09-20: Updated annotations to latest manuscript version
Update 2018-02-16: Separated Diaphragm cells from Muscle cells, and Aorta cells from Heart cells.
Update 2018-02-20: Aorta and Heart erroneously contained Diaphragm and Muscle data, and have now been corrected.
Update 2018-03-09: Renamed tissues for nomenclature standards:
- "Colon" --> "Large_Intestine"
- "Muscle" --> "Limb_Muscle"
- "Mammary" --> "Mammary_Gland"
- "Brain_Microglia" --> "Brain_Myeloid"
- "Brain_Non-microglia" --> "Brain_Non-Myeloid"
Update 2018-03-22: Renamed subtissues:
- tissue: Heart, subtissue: ? --> tissue: Heart, subtissue: Unknown
- tissue: Skin, subtissue: NA --> tissue: Skin, subtissue: Telogen
Update 2018-03-23: Removed row numbers in first column of metadata_FACS.csv
Update 2018-03-27: Added tissue tSNEs and cluster ids