Hepatic SREBP signaling requires SPRING to govern systemic lipid metabolism in mice and humans

The sterol regulatory element binding proteins (SREBPs) are transcription factors that govern cholesterol and fatty acid metabolism. Owing to their central role in hepatic lipid and lipoprotein metabolism their activity is tightly coordinated, and accordingly, dysregulation of the SREBP pathway is associated with the development of, amongst others, cardiovascular and non-alcohol fatty liver disease. Constitutive or inducible global ablation of Spring in mice is lethal, and therefore to interrogate the physiological role of SPRING in hepatic lipid metabolism we developed liver-specific Spring knockout mice (LKO).  In the liver of those mice, shotgun proteomics was assessed in n=6 (pooled together n=3 for each genotype) and injected twice in  Dionex Ultimate 3000 nano-LC system (Sunnyvale CA, USA) connected to an orbitrap Fusion™ Tribrid™ Mass Spectrometer (Thermo Scientific, Bremen, Germany) equipped with a nano-electrospray ion source operating in positive ion mode.