pone.0296350.s004.zip (4.27 MB)
S4 Fig -
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posted on 2024-02-23, 18:48 authored by Ahmed F. A. Ghareeb, James C. Foutz, Gustavo H. Schneiders, Jennifer N. Richter, Marie C. Milfort, Alberta L. Fuller, Romdhane Rekaya, Samuel E. AggreyGenes’ expression level measured by RT-qPCR compared with the fold change produced using Hi-Seq data for the chickens infected with Eimeria maxima raised either under HS (HSi) or thermoneutral condition (TNi) at 6 day-post-treatment (TNi vs. HSi) (A), and the correlation test shows the correlation coefficient (r) of the expression values produced by either method (B). Taking the expression values of the TNi as the control for the relative expression of the HSi group brought the expression values of the TNi group to 1 (Livak’s method). Error bars depict the SEM.
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multiple amino acidscutoff ≥ 135 ° ctnc vs tnitnc vs hsccalcium signaling pathwaylipid metabolic pathwayshost transcriptome responsetni vs hsihsi vs hsc>) maxima cytoplasmic calcium concentrationtwo stressors restoredincluding genes responsiblefatty acid metabolismconsequently might affect3 tnc ), thermoneutralprotein synthesis pathwaysfour treatment groupseimeria maxima tni ), heatmaxima hsi ),hsc ),protein synthesisincluding genestni revealedcalcium availabletreated groupsmight resultindole pathwaysileum transcriptomecytoplasmic poolcombination affectassociated pathwayse type chickenstryptophan metabolismstudy elucidatesseasonal stressorrandomly allocatedpoor performanceheat stressfold changeday postcellular functionscatabolizing tryptophan2304 degs10 birds05 ).
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