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Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.)
dataset
posted on 2016-11-12, 22:47 authored by Gianluca AmorosoGianluca Amoroso, Tomer Ventura, Jennifer Cobcroft, Mark Adams, Abigail Elizur, Chris CarterLower jaw deformity (LJD) is a skeletal anomaly
affecting farmed triploid Atlantic salmon (Salmo salar L.) which leads
to considerable economic losses for industry and has animal welfare
implications. The
aim of this project was to delineate for the first time through transcriptome analysis differential gene
expression in the jaw of fish affected by LJD (compared to normal fish) to detect genes which correlate with and may
characterise LJD, allowing the description of possible mechanisms underlying
the condition.
Lower jaw tissue of triploid Atlantic salmon individuals affected
by LJD (n = 6) and phenotypically normal (Normal) (n = 6) were
used. Equal amounts of RNA from three individuals from the same category were
mixed to generate a total of four pooled samples representing two replicates of
LJD and two replicates of Normal.
Samples were prepared for sequencing by the
Australian Genome Research Facility (AGRF, Melbourne, Australia) according to
the manufacturer’s instructions (Illumina, San Diego, CA, USA).
The files made available are sequencing data in BAM format of the four pooled samples mentioned above. The name of each file allows to easily identify which pooled sample each file corresponds to.
