Photographs of mature male guppies from 5 locations in the Guanapo Tributary on the island of Trinidad. Guppies were experimentally introduced from the Guanapo high predation location into four previously guppy-free low predation reaches of the Upper Lalaja, Lower Lalaja, Taylor, and Caigual Rivers in 2008 or 2009. Photographs were taken in 2008 (Guanapo), 2012 (Upper and Lower Lalaja), or 2013 (Taylor and Caigual) to examine rapid evolution of male color pattern in the introductions compared to their ancestor. Males were anesthetized in MS-222 and photographed in a lab under uniform lighting and photographic conditions. A tab-delimited text key to all photographs has been included.