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Instrument-specific relative units (RU) for CFX96TM (BIO-RAD®) and the corresponding IHC equivalents.

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posted on 2021-09-23, 17:36 authored by Emanuele Caselli, Cristina Pelliccia, Valeria Teti, Guido Bellezza, Martina Mandarano, Ivana Ferri, Kerstin Hartmann, Mark Laible, Ugur Sahin, Zsuzsanna Varga, Chiara Lupi, Fabrizio Stracci, Angelo Sidoni

Instrument-specific relative units (RU) for CFX96TM (BIO-RAD®) and the corresponding IHC equivalents.

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    xlink "> hereinxlink "> determinationxlink "> decadeswider dynamic rangetraditional determination assaystissue block usingquality control effortsmammatyper &# 174least one callone case ).biomarkers expressions closeqpcr may offer96 discrepant casesxlink "> correlationthree study methodssubsequent independent manualrna expression dataimprove ki67 standardizationeither rev alone1 %) casesprinciple method usedeither equivocal resultsqpcr original ihc slidesautomated ihc assessmentsqpcr ).reliable biomarkerscomplementary method2 %)remain equivocalequivocal category28 casesihc ),routine methodsinvestigated discrepancieshigher reproducibilityfindings seemestrogen receptorendocrine responsivenesscomputational pathologycompares wellcategorized differentlybreast cancerbiomarker testing

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