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High-resolution Figure Images for Bloss et al, Neuron (2016)
These are the original high-resolution tiff image files that are pertain to Figures 1-4 and Figures S1-S3 from "Structured dendritic inhibition supports branch-selective integration in CA1 pyramidal cells" by Bloss et al, Neuron (2016).
-Figure 1 and S1 contain images of mouse hippocampal neuropil that was first imaged by array tomography (synaptophyson1 immunoreactivity in blue) then re-imaged with an electron microscope. This correlative imaging allows the accuracy of the light-level data to be measured directly against the TEM ultrastructure. Additional images in Figure S1 include an interneuron axon reconstructed across serial array sections (Panel D) and a reconstructed spine from array tomography and TEM serial sections (Panel E).
-Figures 2-4 contain images from our large-scale array tomography volumes. The image in Figure 2 Panel C is a portion of the volume from the VGAT-ires-Cre array, while those in Figures 3 and 4 are from the array volumes of SST-ires-Cre, nNOS-ires-CreER, and NPY-ires-Cre samples.
-Figure S2 contains confocal images of a fluorescent in situ hybridization experiment (Panel A and B; vgat mRNA, green; gad2 mRNA, red; cre mRNA, blue), while those in Panel D show tiled confocal images of the hippocampal interneurons labeled by virus in each transgenic line. Images related to Panel E show that the Thy1-EGFP pyramidal cells rarely show immunoreactivity for Calbindin1 (a marker of pyramidal cells from the superficial lamina), and those from Panel F show the deep-layer position of Thy1-EGFP soma (green) within the pyramidal cell layer (DAPI in blue) from an array volume.
-Figure S3 Panel A contains array tomography projections of the different branch types present on a pyramidal cell dendritic tree, and Panel C shows a 3D model reconstruction of a single branch.
-Figure 1 and S1 contain images of mouse hippocampal neuropil that was first imaged by array tomography (synaptophyson1 immunoreactivity in blue) then re-imaged with an electron microscope. This correlative imaging allows the accuracy of the light-level data to be measured directly against the TEM ultrastructure. Additional images in Figure S1 include an interneuron axon reconstructed across serial array sections (Panel D) and a reconstructed spine from array tomography and TEM serial sections (Panel E).
-Figures 2-4 contain images from our large-scale array tomography volumes. The image in Figure 2 Panel C is a portion of the volume from the VGAT-ires-Cre array, while those in Figures 3 and 4 are from the array volumes of SST-ires-Cre, nNOS-ires-CreER, and NPY-ires-Cre samples.
-Figure S2 contains confocal images of a fluorescent in situ hybridization experiment (Panel A and B; vgat mRNA, green; gad2 mRNA, red; cre mRNA, blue), while those in Panel D show tiled confocal images of the hippocampal interneurons labeled by virus in each transgenic line. Images related to Panel E show that the Thy1-EGFP pyramidal cells rarely show immunoreactivity for Calbindin1 (a marker of pyramidal cells from the superficial lamina), and those from Panel F show the deep-layer position of Thy1-EGFP soma (green) within the pyramidal cell layer (DAPI in blue) from an array volume.
-Figure S3 Panel A contains array tomography projections of the different branch types present on a pyramidal cell dendritic tree, and Panel C shows a 3D model reconstruction of a single branch.
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