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Haliangium ochraceum Encapsulin:Encapsulated Ferritin size-exclusion chromatography

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posted on 29.09.2021, 08:41 by Jon Marles-WrightJon Marles-Wright
Recombinant Haliangium ochraceum encapsulin and encapsulin:encapsulated ferritin complexes were purified by anion exchange and subjected to size-exclusion chromatography.

Pooled and concentrated samples from the anion exchange step were loaded on a size exclusion chromatography column (Sephacryl 400, Cytiva) equilibrated with SEC buffer (20 mM HEPES, pH 8.0, 150 mM NaCl).

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Understanding iron acquisition within a bacterial iron-megastore

Biotechnology and Biological Sciences Research Council

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