Human Airway Epithelial Cell (HAEC) from a single donor were grown in triplicate and harvested on days 0, 2, 3, 7, 14, 21 and 28. Significant increases were observed in a) trans-epithelial resistance (TEER) b) MUC5AC mucin mRNA production over the time course, indicating full HAEC differentiation. Error bars indicate standard errors of the mean.