Free hemoglobin determination atpatients’ bedside to evaluate hemolysis: supplementary data

Background: The authors report the relevance of using a point of care test (Helge R ? ) for free hemoglobin

determination and concordance of the values the with Cobas R ? 8000 and spectrophotometer methods.

Results: The within-run of the point of care test was <3%. Good correlations among the three

methods were observed and an acceptable concordance for hemolysis index values from 50 mg/dl.

An excellent agreement between the Cobas 8000 and the spectrophotometer was found. Conclusion:

Automated methods represent methods of choice for free hemoglobin determination. An advantage

of the Helge system is that it can be applied to samples experiencing a delay in evaluation due to the

long distance between the collection site and the central laboratory. Another advantage is its use at the

bedside, in the monitoring of extracorporeal membrane oxygenation patients.