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Drug-resistance associated mutations in P. falciparum genes crt, mdr1, dhps, and dhfr.

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posted on 2022-01-14, 18:26 authored by Swarnali Louha, Camelia Herman, Mansi Gupta, Dhruviben Patel, Julia Kelley, Je-Hoon M. OH, Janani Guru, Jean F. Lemoine, Michelle A. Chang, Udhayakumar Venkatachalam, Eric Rogier, Eldin Talundzic

Drug-resistance associated mutations in P. falciparum genes crt, mdr1, dhps, and dhfr.

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    values (+/- 1time saving approachproviding allele frequencymedian codon coveragedried blood spotsdiv >< pcontinued chloroquine sensitivitycompared codon coverageμl blood showeddrug resistant markersplasmodium falciparum μl blood priorsequencing large numbersdeep sequencing yieldedlower parasite densityaf estimation may0 ct valuepooled sequencing approachdrug resistance genesseveral individual samplesμl priordrug resistancedeep sequencingsequencing successlarge numbergenes (<mdr1 dhps dhfr crt similar realrapid genotypingpooled samplesparasite dnaoften neededmolecular surveillancemixed infectionslow amountsindividual samplesgrouping samplescould leadconferring mutationsbased studiesassociated mutationsalternative costaf calls40 parasites

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