Drought adaptation modifies soil respiration and microbial community responses to phytohormones

Datasets used in:

Sayer EJ, Crawford JA, Edgerley J, Askew AP, Hahn CZ, Whitlock R and Dodd IC (2021) Adaptation to chronic drought modifies soil microbial community responses to phytohormones. Communications Biology

MS COMMSBIO-20-2294A

File 1: SayerEtAl2021_CommsBiol_SoilResp.csv

Respiration rates (CO₂ efflux) following phytohormone additions at three molar concentrations or a standard root exudate solution, applied to soils from long-term climate change treatments at the Buxton Climate Change Impacts Study, UK. Respiration rates were measured using the Microresp method during 6-h incubations.

Column headings:

Block -> replicate block, determined by original experimental design in the field

Climate.Plot -> climate treatment applied to field plots

RE.add -> addition of standard root exudate solution (RE) or no addition (noRE)

Hormone.Trt -> phytohormone treatment applied to the soils, where ABA is abscisic acid, ACC is 1-aminocyclopropane-1-carboxylic acid and JA is jasmonic acid

Concentration-> molar concentration of the phytohormone solutions applied to soils, where 0M is a procedural control (deionised water)

CO2efflux->total soil respiration in micrograms (ug) C

File 2: SayerEtAl2021_CommsBiol_PLFAs.csv

Soil properties and soil microbial biomarker groups in field soils (inc.Time = 0) and soil microbial biomarker groups after 24h incubation with phytohormone additions at 1M concentration; soils were sampled from long-term climate change treatments at the Buxton Climate Change Impacts Study, UK; biomarker functional groups were determined by phospholipid fatty acid analysis and are given as relative abundances.

Column headings:

Block -> replicate block, determined by original experimental design in the field

Climate.Plot -> climate treatment applied to field plots

Hormone.Trt -> phytohormone treatment applied to the soils at 1M concentration, where ABA is abscisic acid, ACC is 1-aminocyclopropane-1-carboxylic acid and JA is jasmonic acid; CT is field soils before incubation and H2O is a procedural control (incubated with deionised water only).

Inc.Time -> incubation time in hours, where 0 is field soils (pre-incubation)

Total.Biomass -> total PLFA biomass in nM/g soil

AM.Fungi-> arbuscular mycorrhizal fungi (% relative abundance)

Gram.Negative -> Gram negative bacteria (% relative abundance)

Eukaryote-> Eukaryotes (% relative abundance)

Fungi -> saprophytic fungi (% relative abundance)

Gram.Positive -> Gram positive bacteria (% relative abundance)

Actinomycetes -> Actinomycetes (% relative abundance)

FBratio -> Fungal to bacterial ratio

nMfungi -> saprophytic fungal biomass in nM/g soil

nMbacteria -> bacterial biomass in nM/g soil

Gram.Ratio -> ratio of Gram positive to Gram negative bacteria

SWC.percent -> soil water content (%) at the time of sampling

ExtrC.mg.g -> K2SO4-extractable soil carbon (mg/g dry weight)

ExtrN.mg.g-> K2SO4-extractable soil nitrogen (mg/g dry weight)