Diamond annotation

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Annotation list for Calanus finmarchicus reference transcriptome using DIAMOND³.

Contigs were aligned with DIAMOND??³ on NR (2019-09-29), Swissprot and Trembl (2018-12) to retrieve corresponding best annotations.

An annotation matrix was then generated by selecting the best hit for each database if: i) the percent of the query length covered by the alignment was higher than 60% ; ii) the percent of the subject length covered by the alignment was higher than 40%; iii) the percent of identity of the alignment was higher than 40%.

File diamond_annotation_206k.tsv is the annotation list for Calanus finmarchicus reference transcriptome using DIAMOND³ (36,274 contigs with an annotation in at least one database out of the 206,012).

File diamond_annotation_76k.tsv is the annotation list for the 76,550 contigs expressed with more than 1 CPM in the RNA sequencing Bioproject PRJNA628886 using DIAMOND³ (22,527 contigs with an annotation in at least one database out of the 76,550).

³DIAMOND: version v0.9.22, parameters: -f 6 qseqid qlen qcovhsp pident score evalue length sseqid slen stitle.

Ref: Buchfink, B., Xie, C. & Huson, D. H. Fast and sensitive protein alignment using DIAMOND. Nat. Methods 12, 59–60 (2015).