Density experiment in Negev Desert Israel

<p>1 Few experiments directly test the role of density dependence in natural plant communities.<br>2 We tested (i) whether different initial seed densities of the entire seed bank of an annual plant community affected performance (emergence, mean plant biomass and survival) and (ii) whether density-dependent processes were operating within the communities. We also tested whether life-stage, year and soil moisture (both on a topographic gradient and in an experimental manipulation) influenced the effects of seed density.</p> <p>3 We considered two distinct phases: seed to emergent seedlings, and emergent seed- lings to established plants.<br>4 The seed bank was collected from a semistabilized sand dune in the Negev Desert, Israel. This was added to sieved sand in plots at the same site at four different initial seed densities: 1/16×, 1/4×, 1× (natural seed density) and 2×. The experiment was repeated for three consecutive growing seasons.</p> <p>5 Emergence of seedlings was significantly influenced by initial seed density in all 3 years, with higher initial seed densities having lower rates of emergence.<br>6 Mean final plant size was negatively density dependent and consistently unaffected by the initial seed density sown.</p> <p>7 In general, there were no strong interactions of topographic position with initial seed density, processes within the vegetation were not density dependent and the experi- mental addition of water did not influence any of the performance measures tested. Density-dependent processes vary from year to year, while moisture effects do not.</p> <p>8 We conclude that seedling emergence and some processes in the established plant community are density dependent, but the established plant community is also affected by other processes, such as resource limitation.</p> <p> </p>