Data_Sheet_1_Biological Characterization of Yellow Fever Viruses Isolated From Non-human Primates in Brazil With Distinct Genomic Landscapes.pdf (917.11 kB)

Data_Sheet_1_Biological Characterization of Yellow Fever Viruses Isolated From Non-human Primates in Brazil With Distinct Genomic Landscapes.pdf

dataset

posted on 2022-02-14, 04:43 authored by Nathália Dias Furtado, Lidiane de Menezes Raphael, Ieda Pereira Ribeiro, Iasmim Silva de Mello, Déberli Ruiz Fernandes, Mariela Martínez Gómez, Alexandre Araújo Cunha dos Santos, Mônica da Silva Nogueira, Márcia Gonçalves de Castro, Filipe Vieira Santos de Abreu, Lívia Carício Martins, Pedro Fernando da Costa Vasconcelos, Ricardo Lourenço-de-Oliveira, Myrna Cristina Bonaldo

Since the beginning of the XXI Century, the yellow fever virus (YFV) has been cyclically spreading from the Amazon basin to Brazil’s South and Southeast regions, culminating in an unprecedented outbreak that started in 2016. In this work, we studied four YFV isolated from non-human primates obtained during outbreaks in the states of Rio Grande do Sul in 2008 (PR4408), Goiás (GO05), and Espírito Santo (ES-504) in 2017, and Rio de Janeiro (RJ 155) in 2019. These isolates have genomic differences mainly distributed in non-structural proteins. We compared the isolates’ rates of infection in mammal and mosquito cells and neurovirulence in adult mice. RJ 155 and PR4408 YFV isolates exhibited higher infectivity in mammalian cells and neurovirulence in mice. In mosquito Aag2 cells, GO05 and PR4408 displayed the lowest proliferation rates. These results suggest that RJ 155 and PR4408 YFV isolates carry some genomic markers that increase infectivity in mammal hosts. From this characterization, it is possible to contribute to discovering new molecular markers for the virulence of YFV.