DNA markers used for fine mapping spring1 on chromosome 7.

DNA markers from the MtCO region of chromosome 7 were analysed for linkage to spring1 in the mapping crosses (see Text). The interval containing spring1 is flanked by Medtr7g084170.1 and Medtr7g085190.1 shown in bold; each marker is separated from spring1 by one recombination event.

DNA marker taken from the University of Minnesota (UMN) Integrated Genetic Map of Medicago truncatulahttp://www.medicago.org/genome/map.php.

DNA marker from Pierre et al (2010).

Size of PCR fragment predicted from Mt3.5 Genome assembly from http://medicagohapmap.org/.

Numbers of recombinants detected by the marker in the late or early flowering classes from the F2 of the “spring1 x Jester” cross, or from the Test cross (see Table 1 and Text). “Test Cross” is (♂(♂spring1 x ♀Jester) x ♀Jester). NT is not tested. Indel is insertion/deletion; SSR is Simple Sequence Repeat. The column entitled Mt3.5 Genome assembly gives the position of the marker on the pseudomolecule from Mt3.5 Genome assembly from http://medicagohapmap.org/after a Chromosome Visualisation Tool (CViT) BLAST search http://medicagohapmap.org/was carried out.