Comparison of efficiency, limit of quantification and limit of detection in the two different duplex probe-based qPCR set-ups.

For each of the two primer/probe combinations the efficiency, limit of quantification and limit of detection were assessed using a 10-fold dilution series of a standard fragment (10⁸−10⁰ copies/μl). The choice for these specific combinations of primer pairs for both genes was based upon limited primer/probe interactions.